Activation of Proteasome in Sea Urchin Sperm by Lysophosphatidylinositol and by Sperm Lipids. (proteasome/sea urchin/sperm/acrosome reaction/lysophospholipid)
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چکیده
منابع مشابه
Inhibition and relaxation of sea urchin sperm flagella by vanadate
Direct measurements of the stiffness (elastic bending resistance) of demembranated sera urchin sperm flagella were made in the presence of MgATP2- and vanadate. Under these conditions, the flagellum is in a relaxed state, with a stiffness of approximately 0.9 x 10(-21) N m2, which is approximately 5% of the stiffness obtained in the rigor state in the absence of MgATP2-. MgADP- dose not substit...
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The Lubrol-dispersed guanylate cyclase from sea urchin sperm was purified and isolated essentially free of detergent by GTP affinity chromatography, DEAE-Sephadex chromatography, and gel filtration. After removal of the detergent, the enzyme remained in solution in the presence of 20% glycerol. The specific activity of the purified enzyme was about 12 pmol of guanosine 3’:5’-monophosphate (cycl...
متن کاملStarting transients in sea urchin sperm flagella
Live sea urchin spermatozoa were rendered immotile by lowered pH; Triton-extracted spermatozoa were rendered immotile by either lowered pH or by deprivation of ATP. The spermatozoa began to beat after an increase in pH or as ATP was supplied, and the first bends were recorded on ciné film. Triton-extracted spermatozoa deprived of ATP retained a partially formed basal bend which could be either ...
متن کاملSperm-oocyte interaction in the sea-urchin.
A study was made of the electrical and morphological changes in the sea-urchin oocyte during interaction with spermatozoa. The first event, a small step depolarization accompanied by a 20-40% decay in input resistance occurs within seconds of attachment. The evidence suggests that this electrical event is the result of sperm-oocyte fusion, and that the ion channels that lower the resistance acr...
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Sperm of the sea urchin Tripneustes gratilla repeatedly start and stop swimming when suspended in seawater and observed by dark-field microscopy. While in the quiescent state, which usually lasts about a second, the sperm assume s shape resembling a cane, with a sharp bend of approximately 3.4 rad in the proximal region of the flagellum and very little curvature in the rest of the flagellum exc...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1991
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1991.00259.x